Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
Identifieur interne : 003700 ( Main/Exploration ); précédent : 003699; suivant : 003701Generic detection of coronaviruses and differentiation at the prototype strain level by reverse transcription-PCR and nonfluorescent low-density microarray.
Auteurs : Luciano Kleber De Souza Luna [Allemagne] ; Volker Heiser ; Nicolas Regamey ; Marcus Panning ; Jan Felix Drexler ; Sabue Mulangu ; Leo Poon ; Sigrid Baumgarte ; Bert Jan Haijema ; Laurent Kaiser ; Christian DrostenSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2007.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), Coronavirus (), Coronavirus (génétique), Coronavirus (isolement et purification), Coronavirus humain 229E (), Coronavirus humain 229E (génétique), Coronavirus humain 229E (isolement et purification), Coronavirus humain OC43 (), Coronavirus humain OC43 (génétique), Coronavirus humain OC43 (isolement et purification), Humains, RT-PCR (), Spécificité d'espèce, Séquençage par oligonucléotides en batterie (), Techniques de typage bactérien, Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ARN viral.
- génétique : ARN viral, Coronavirus, Coronavirus humain 229E, Coronavirus humain OC43, Virus du SRAS.
- isolement et purification : Coronavirus, Coronavirus humain 229E, Coronavirus humain OC43, Virus du SRAS.
- Coronavirus, Coronavirus humain 229E, Coronavirus humain OC43, Humains, RT-PCR, Spécificité d'espèce, Séquençage par oligonucléotides en batterie, Techniques de typage bactérien, Virus du SRAS.
English descriptors
- KwdEn :
- Bacterial Typing Techniques, Coronavirus (classification), Coronavirus (genetics), Coronavirus (isolation & purification), Coronavirus 229E, Human (classification), Coronavirus 229E, Human (genetics), Coronavirus 229E, Human (isolation & purification), Coronavirus OC43, Human (classification), Coronavirus OC43, Human (genetics), Coronavirus OC43, Human (isolation & purification), Humans, Oligonucleotide Array Sequence Analysis (methods), RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (classification), SARS Virus (genetics), SARS Virus (isolation & purification), Species Specificity.
- MESH :
- chemical , analysis : RNA, Viral.
- classification : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, SARS Virus.
- genetics : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, RNA, Viral, SARS Virus.
- isolation & purification : Coronavirus, Coronavirus 229E, Human, Coronavirus OC43, Human, SARS Virus.
- methods : Oligonucleotide Array Sequence Analysis, Reverse Transcriptase Polymerase Chain Reaction.
- Bacterial Typing Techniques, Humans, Species Specificity.
Abstract
A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.
DOI: 10.1128/JCM.02426-06
PubMed: 17229859
Affiliations:
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Le document en format XML
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SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">A nonfluorescent low-cost, low-density oligonucleotide array was designed for detecting the whole coronavirus genus after reverse transcription (RT)-PCR. The limit of detection was 15.7 copies/reaction. The clinical detection limit in patients with severe acute respiratory syndrome was 100 copies/sample. In 39 children suffering from coronavirus 229E, NL63, OC43, or HKU1, the sensitivity was equal to that of individual real-time RT-PCRs.</div></front></TEI><affiliations><list><country><li>Allemagne</li></country><region><li>Hambourg</li></region><settlement><li>Hambourg</li></settlement></list><tree><noCountry><name sortKey="Baumgarte, Sigrid" sort="Baumgarte, Sigrid" uniqKey="Baumgarte S" first="Sigrid" last="Baumgarte">Sigrid Baumgarte</name><name sortKey="Drexler, Jan Felix" sort="Drexler, Jan Felix" uniqKey="Drexler J" first="Jan Felix" last="Drexler">Jan Felix Drexler</name><name sortKey="Drosten, Christian" sort="Drosten, Christian" uniqKey="Drosten C" first="Christian" last="Drosten">Christian Drosten</name><name sortKey="Haijema, Bert Jan" sort="Haijema, Bert Jan" uniqKey="Haijema B" first="Bert Jan" last="Haijema">Bert Jan Haijema</name><name sortKey="Heiser, Volker" sort="Heiser, Volker" uniqKey="Heiser V" first="Volker" last="Heiser">Volker Heiser</name><name sortKey="Kaiser, Laurent" sort="Kaiser, Laurent" uniqKey="Kaiser L" first="Laurent" last="Kaiser">Laurent Kaiser</name><name sortKey="Mulangu, Sabue" sort="Mulangu, Sabue" uniqKey="Mulangu S" first="Sabue" last="Mulangu">Sabue Mulangu</name><name sortKey="Panning, Marcus" sort="Panning, Marcus" uniqKey="Panning M" first="Marcus" last="Panning">Marcus Panning</name><name sortKey="Poon, Leo" sort="Poon, Leo" uniqKey="Poon L" first="Leo" last="Poon">Leo Poon</name><name sortKey="Regamey, Nicolas" sort="Regamey, Nicolas" uniqKey="Regamey N" first="Nicolas" last="Regamey">Nicolas Regamey</name></noCountry><country name="Allemagne"><region name="Hambourg"><name sortKey="De Souza Luna, Luciano Kleber" sort="De Souza Luna, Luciano Kleber" uniqKey="De Souza Luna L" first="Luciano Kleber" last="De Souza Luna">Luciano Kleber De Souza Luna</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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